NGS: ATAC-seq

Turn ATAC-seq data into meaningful biological insight by learning how to analyse peaks, annotate features and assess differential accessibility.

3 hours

Basics of NGS

Intermediate

Course information

This course provides a practical introduction to the analysis and visualisation of ATAC-seq data. Building on the foundations of Next-Generation Sequencing, it will help you understand how ATAC-seq can be used to explore chromatin accessibility and generate meaningful insights for biomedical research, moving from processed NGS outputs to biological interpretation.

Through flexible, hands-on learning in R and RStudio, you will read ATAC-seq data, annotate genomic features, visualise results, call peaks and perform differential accessibility analysis.

By the end of the course, you will understand what ATAC-seq data can offer, how bulk ATAC-seq analysis is carried out, and how to apply these methods using example datasets prepared through established NF-core ATAC-seq pipelines.

Learning objectives

  • Obtain a clear understanding of how ATAC-seq data are analysed and visualised
  • Understand how peaks are called
  • Perform differential accessibility test
  • Use R to read NGS data and annotate features